LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1862..1958) /label=attL2 /note="attL2 (c)" misc_signal complement(1973..1992) /note="T7 Promoter/priming site (c)" primer_bind 2000..2016 /note="M13 Reverse priming site" gene 2129..2938 /note="Kanamycin resistance gene" rep_origin 3059..3732 /note="pUC origin" vector join(1870..3735,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1855 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:27060 IMAGE:4814499" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_95" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..1855 /gene="CHST1" /gene_synonym=C6ST /gene_synonym=KS6ST /gene_synonym=KSGal6ST /db_xref="GeneID:8534" /db_xref="HGNC:1969" /db_xref="MIM:603797" CDS 692..1855 /dnas_title="carbohydrate (keratan sulfate Gal-6) sulfotransferase 1" /gene="CHST1" /gene_synonym=C6ST /gene_synonym=KS6ST /gene_synonym=KSGal6ST /codon_start=1 /product="carbohydrate (keratan sulfate Gal-6) sulfotransferase 1" /protein_id="AAH22567.1" /db_xref="GI:21961240" /db_xref="GeneID:8534" /db_xref="HGNC:1969" /db_xref="MIM:603797" /translation="MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAE RLCEESPTFAYNLSRKTHILILATTRSGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIP RFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIKPPPVNHTTDRIFRRGASRVL CSRPVCDPPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRA LVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVC EDFSNSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQN NTRGDPTLGKHKYGTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIAASEE ELKNPSVSLVEERDFRPFS" misc_difference 835..835 /gene="CHST1" /gene_synonym=C6ST /gene_synonym=KS6ST /gene_synonym=KSGal6ST /note="'C' in cDNA is 'T' in the human genome; no amino acid change." misc_feature 692..1855 /note="CHST1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccgcaccttc accgccaagt cctttcacac 721 ctgccccggg ctggcagagg ccgggctggc cgagcgactg tgcgaggaga gccccacctt 781 cgcctacaac ctctcccgca agacccacat cctcatcctg gccaccacgc gcagcggctc 841 ctccttcgtg ggccagctct tcaaccagca cctggacgtc ttctacctgt ttgagcccct 901 ctaccacgtc cagaacacgc tcatcccccg cttcacccag ggcaagagcc cggccgaccg 961 gcgggtcatg ctaggcgcca gccgcgacct cctgcggagc ctctacgact gcgacctcta 1021 cttcctggag aactacatca agccgccgcc ggtcaaccac accaccgaca ggatcttccg 1081 ccgcggggcc agccgggtcc tctgctcccg gcctgtgtgc gaccctccgg ggccagccga 1141 cctggtcctg gaggaggggg actgtgtgcg caagtgcggg ctactcaacc tgaccgtggc 1201 ggccgaggcg tgccgcgagc gcagccacgt ggccatcaag acggtgcgcg tgcccgaggt 1261 gaacgacctg cgcgccctgg tggaagaccc gcgattaaac ctcaaggtca tccagctggt 1321 ccgagacccc cgcggcattc tggcttcgcg cagcgagacc ttccgcgaca cgtaccggct 1381 ctggcggctc tggtacggca ccgggaggaa accctacaac ctggacgtga cgcagctgac 1441 cacggtgtgc gaggacttct ccaactccgt gtccaccggc ctcatgcggc ccccgtggct 1501 caagggcaag tacatgttgg tgcgctacga ggacctggct cggaacccta tgaagaagac 1561 cgaggagatc tacgggttcc tgggcatccc gctggacagc cacgtggccc gctggatcca 1621 gaacaacacg cggggcgacc ccaccctggg caagcacaaa tacggcaccg tgcgaaactc 1681 ggcggccacg gccgagaagt ggcgcttccg cctctcctac gacatcgtgg cctttgccca 1741 gaacgcctgc cagcaggtgc tggcccagct gggctacaag atcgccgcct cggaggagga 1801 gctgaagaac ccctcggtca gcctggtgga ggagcgggac ttccgcccct tctcgTAGGA 1861 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 1921 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 1981 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2041 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2101 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2161 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2221 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2281 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2341 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2401 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2461 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2521 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2581 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2641 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2701 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2761 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2821 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 2881 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 2941 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3001 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3061 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3121 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3181 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3241 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3301 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3361 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3421 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3481 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3541 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3601 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3661 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3721 TTTTGCTCAC ATGTT //